Trichosporon asteroides is an asexual basidiomycetous fungus first described from human skin but is now mainly isolated from blood and urine. A fatal case of trichosporon asahii fungemia and pneumonia. A rare case report by shihta shang, yasung yang and mingyieh peng download pdf 183 kb. For many years, the unique species of the genus, trichosporon beigelli, was only known as an environmental and saprophytic fungus occasionally found as the etiological agent of white piedra.
Trichosporon asahii candida albicans candida parasilopsis unclassified ascomycota family nectriaceae gibberella zeae hypocreales sp. The fungal genus trichosporon contains emerging opportunistic pathogens of humans, and is the third most commonly isolated noncandidal. Neutropenia developing due to malignancies is an important risk factor for fungal infection. Trichosporon species isolated from the perigenital region. Superficial cutaneous trichosporon asahii infection in an immunocompetent host. However, case reports of invasive trichosporonosis have been frequently published and the genus is. Full text a fatal case of trichosporon asahii fungemia and. In this study, six strains, including two clinical isolates, from four different species trichosporon asahii, t. Feb 12, 2018 a fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit.
The aim of the present study was to evaluate the antifungal susceptibility profile of trichosporon species isolated from different sources employing the clinical and laboratory standards institute clsi method and etest method. Each strain produced a brownish pigment compatible with melanin. Abstractthe present study aimed to 1 determine the colonization rates of medically important. This system utilized 2 pantrichosporon probes designed to hybridize to the its2 region, which is highly variable among the genomes of individual fungal species, and it detected at least four clinically relevant trichosporon species t. There are only sporadic reports of infections caused by t. Feb 12, 2018 all pathogenic members of the genus trichosporon were once regarded as a single species, t beigelii. Antachopoulos c, papakonstantinou e, dotis j, bibashi e, tamiolaki m, koliouskas d. Invasive trichosporonosis caused by trichosporon asahii and other unusual trichosporon species at a medical center in taiwan. Trichosporon inkin as an emergent pathogen in patients. Clinical breakpoints have not been defined for trichosporon species, and the mics cannot be reliably interpreted as indicating resistance or susceptibility. Jul 19, 2019 asahii strain jcm 2466 was used instead of the sequence for strain cbs 2479. Trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen seen particularly in immunologically compromised patients. Primary cutaneous trichosporonosis responsive to voriconazole. An trichosporon asahii in nahilalakip ha genus nga trichosporon, ngan familia nga trichosporonaceae.
In our study, resistance to fluconazole was detected. Proliferation of trichosporon yeasts in the hair can lead to an unpleasant but nonserious condition. A 67 year old male presented with a widespread erythematous rash prior to sustaining a syncopal episode outside the dermatologistsof. Isolation and antifungal susceptibility testing of. Trichosporon asahii is an opportunistic fungus that causes infections in immunosuppressed patients. A fatal outbreak of trichosporon asahii sepsis in a neonatal intensive care unit. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin. Trichosporon asahii infection in an advanced aids patient.
Biofilm formation leads to antifungal drug resistance, so biofilmrelated infections are relatively difficult to treat and infected devices often require surgical removal. First genotype identification of trichosporon asahii in. A fatal case of trichosporon asahii fungemia and pneumonia in. Text is available under the creative commons attributionsharealike license. Cmi gml n caso agente fecha cultivo recuento ufcml anfotericina b ketoconazol itraconazol fluconazol 1 t. Invasive trichosporonosis is an extremely rare mycosis, but trichosporon fungemia tf in patients with hematologic malignancies has been increasingly recognized to be a fulminant and highly lethal infection. Trichosporon asahii was the main causative species 46. Trichosporon genus is subdivided into six distinct human pathogenic species of which t. Trichosporon asahii was identified from blood cultures, confirmed by morphological examination on cornmeal agar and speciated by biochemical analysis api 20c.
Trichosporon asahii, urinary tract infection trichosporon behrend is a genus of anamorphic yeasts yeast can be isolated from the sputum, the urine, the skin and basidiomycota, hymenomycetes, trichosporonales with the blood. However, case reports of invasive trichosporonosis have been frequently published and the. May 06, 2014 a fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. A fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2. Full text a fatal case of trichosporon asahii fungemia. Two case presentations infected by trichosporon asahii and. Trichosporon asahii as an emerging etiologic agent of disseminated trichosporonosis. The most commonly isolated trichosporon species was trichosporon asahii 57 of 76, 75%. Trichosporon asahii infection with rapid diagnosis.
Data from antifungal susceptibility testing ast studies were also. Trichosporon asahii the most common cause of disseminated disease, trichosporon inkin the cause. Trichosporon asahii is a rare cause of disseminated fungal infection in immunocompromised patients, presenting a diagnostic and therapeutic challenge. Integrated transcriptomic analysis of trichosporon asahii uncovers the core genes and pathways of fluconazole resistance. If you do not see its contents the file may be temporarily unavailable at the journal website or you do not have a pdf plugin installed and enabled in your browser. Leptosphaerulina chartarum unclassified fungi order capnodiales cladosporium herbarum rhodosporidium diobovatum trichosporon sp.
Susceptibility tests were conducted by disk diffusion and broth microdilution. The virulence attributes of trichosporon asahii are virtually unknown, despite its growing relevance as causative agent of superficial and invasive diseases in humans. Trichosporon asahii sepsis in a patient with pediatric malignancy by aslinur ozkayaparlakay, eda karadagoncel, ali bulent cengiz, ates kara, atilla yigit, safak gucer and deniz gur get pdf 541 kb. Abstractthe fungal genus trichosporon contains emerging opportunistic pathogens of humans, and is the third most. Gene ortholog relationships across trichosporon genomes were. Differentiation of the emerging human pathogens trichosporon. Three contigs assembled from the trichosporon reads. Antifungal susceptibility profile of trichosporon isolates. The basidiomycetous yeast, trichosporon behrend, is a medically important genus that includes the causative agents of white piedra in immunocompetent hosts and disseminated infections in immunocompromised hosts. Invasive trichosporonosis caused by trichosporon asahii and. Mar 10, 2020 trichosporon asahii is the major causative fungus of disseminated or deepseated trichosporonosis and forms a biofilm on medical devices. A woman in her 20s presented with a large erythematous indurated plaque involving the face and neck that had been first noted at age 2 years.
Superficial cutaneous trichosporon asahii infection in an. Free fulltext pdf articles from hundreds of disciplines, all in one place differentiation of the emerging human pathogens trichosporon asahii and trichosporon asteroides from other pathogenic yeasts and moulds by using speciesspecific monoclonal antibodies pdf paperity. Infeccion urinaria nosocomial por trichosporon asahii. Melanization of members of the genus trichosporon is poorly described. The patient was treated with amphotericin b and fluconazole, but t. The plaque progressively increased in size, and similar plaques developed over the back, right dorsal surface of the hand, suprapubic area figure 1a, and right thigh over the last 4 years. Fungi of the genus trichosporon are increasingly recognized as causative agents of superficial and invasive fungal disease in humans. Trichosporon asahii and trichosporon asteroides from plos. Although the utility of azole therapy has been demonstrated in several observations, little is known about the efficacy of one of azoles, miconazole mcz. Trichosporon beigelii is a fungus once thought to cause only superficial infections, but recently has been increasingly identified as an opportunistic systemic pathogen in immunocompromised patients. Although most species are considered commensals of the human skin and gastrointestinal tract, these basidiomycetes are an increasing cause of fungal disease among immunocompromised hosts, such as hematological patients and solid organ transplant recipients. First genotype identification of trichosporon asahii in sfax.
Since only sporadic cases of breakthrough trichosporonosis have been reported. Superficial cutaneous trichosporon asahii infection in an immunocompetent host superficial cutaneous trichosporon asahii infection in an immunocompetent host pulvirenti, nella. Pdf trichosporon asahii formerly trichosporon beigelii is an emerging fungal pathogen. Trichosporon asahii, a noncandida yeast that caused fatal. Most are typically isolated from soil, but several species occur as a natural part of the skin microbiota of humans and other animals. Trichosporon asahii an overview sciencedirect topics. Structural and functional properties of the trichosporon. Successful treatment of breakthrough disseminated trichosporon.
Utility of miconazole therapy for trichosporon fungemia in. There are now 100 reported cases of hematogenously disseminated infections with this lifethreatening yeast, and no effective antifungal therapy is available. In vitro antifungal susceptibility testing of the isolates showed that voriconazole exhibited the highest activity. Trichosporon spp trichosporon species are an uncommon but important cause of deep and disseminated infection. Pulvirenti n, dalloglio f, greco am, oliveri s, schwartz ra, micali g. Trichosporon asahii sepsis in a patient with pediatric. This article is from brazilian journal of microbiology, volume 41. Trichosporon asahii, a non candida yeast that caused. Oct 17, 2016 trichosporon asahii was the main causative species 46.
Biochemical and morphologic differences within the genus have led to the division of the former t beigelii into distinct species, at least 9 of which have the potential to cause human disease. We present two pediatric oncology cases successfully treated with voriconazole after t. Trichosporon asahii among intensive care unit patients at a medical center in jamaica volume 34 issue 6 robyn neblett fanfair, orville heslop, kizee etienne, lois rainford, monika roy, lalitha gade, joyce peterson, heather oconnell, judith noblewang, s. We noted a good evolution and disappearance of the casts after the treatment. A fatal case of trichosporon asahii fungemia and pneumonia in a kidney transplant recipient during caspofungin treatment meifang yang,1,2 hainv gao,1,2 lanjuan li1,2 1state key laboratory for diagnosis and treatment of infectious diseases, the first affiliated hospital, school of medicine, zhejiang university. Genomics and transcriptomics analyses of the oilaccumulating. Nov 1, 2017 fungi reads were from hortaea werneckii, aspergillus. Molecular identification was carried out by analysing the igs1 regions of the rdna of 30 trichosporon isolates. Furthermore, four genotypes were identified in tunisia. The identity of all trichosporon species was confirmed by analysis of the intergenic spacer 1 region of the fungal ribosomal rna gene. Glucuronoxylomannan gxm is a well described virulence factor of pathogenic species in the cryptococcus genus. Glucuronoxylomannan gxm is a well described virulence factor of pathogenic species in the cryptococcus.
Trichosporon asahii sepsis in a patient with pediatric malignancy. Trichosporonosis definition of trichosporonosis by medical. Trichosporon mycotoxinivorans, a novel respiratory pathogen in patients with cystic fibrosis. Trichosporon asahii infection with rapid diagnosis mafiadoc. Methods for the diagnosis of invasive trichosporonosis include pcrbased methods, luminex xmap technology. This system utilized 2 pan trichosporon probes designed to hybridize to the its2 region, which is highly variable among the genomes of individual fungal species, and it detected at least four clinically relevant trichosporon species t. By aslinur ozkayaparlakay, eda karadagoncel, ali bulent cengiz, ates kara, atilla yigit, safak gucer and deniz gur. Genome and transcriptome evolve separately in recently. Nosocomial trichosporon asahii fungemia in a patient with secondary hemochromatosis.
In vitro antifungal susceptibilities of trichosporon species. Trichosporon asahii is the major causative fungus of disseminated or deepseated trichosporonosis and forms a biofilm on medical devices. Therefore, prevention of biofilm formation is important in clinical settings. Trichosporon is a genus of anamorphic fungi in the family trichosporonaceae. By multivariate analysis, breakthrough infection or 2. Gxm is also produced by species of the trichosporon genus, and both polysaccharides share antigenic determinants. Trichosporon asahii was the most frequent species detected.
Molecular identification of trichosporon species pdf paperity. All species of trichosporon are yeasts with no known teleomorphs sexual states. Homologs for the pheromone genes were also not found. Molecular targets and identification of trichosporon species in cultures 692. Scarring developed in the plaques over the face and hand during. An trichosporon asahii in uska species han fungi in nahilalakip ha divisio nga basidiomycota, ngan nga ginhulagway ni akagi, sugita, a. The objectives of our study were species identification and genotyping of trichosporon isolates collected at the parasitology and mycology laboratory in sfax, tunisia. Disseminated infection due to trichosporon species is one of the emerging mycoses in neutropenic patients, particularly when they are treated for haematological malignancy. Trichosporon species, another yeast, have been reported as a cause of fungemia, especially in patients who have neutropenia and cancer. A case report and an update a chowdhary, s ahmad, zu khan, dc doval, hs randhawa department of medical mycology, vallabhbhai patel chest institute, university of delhi, new delhi 110 007, india. Gene ortholog relationships across trichosporon genomes were determined using inparanoid version 4.
Molecular identification of trichosporon species pdf. Despite the increased frequency and severity of trichosporonosis, data on the antifungal susceptibilities of trichosporon spp. Infections by this species usually respond to treatment with azoles and amphotericin b. Integrated transcriptomic analysis of trichosporon asahii. Fungemia due to trichosporon asahii in a neutropenic child refractory to amphotericin b.
Although trichosporon is a causative pathogen of white piedra and summer. Trichosporon inkin as an emergent pathogen in patients with. In 1902, vuillemin designated all trichosporon species trichosporon beigelii. To assess its therapeutic role, we retrospectively investigated 6 cases of tf in patients with acute leukemia receiving mcz containing regimens. Frontiers the cell biology of the trichosporonhost. A chowdhary, s ahmad, zu khan, dc doval, hs randhawa. Trichosporonosis definition of trichosporonosis by. The patient was advised to keep the hair dry, and was treated with 2% ketoconazole shampoo topically daily for 2 months with oral fluconazole for 3 months.
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